| Abstract |
Some cell lines originated from M.brassicae are able to be subcultured
with an autoclaved serum-free medium. The medium was modified to omit
components of sodium hydrogen carbonate, sodium dihydrogen phosphate and
serum. These cell lines were SES-MaBr-1, -2, -3, -4 (from fat cells),
NIAS-MaBr-92, -93 (from hemolymph), NIAS-MaBr-32 (from ovary). An inoculum
of 2x10^5 cells/ml of each cell line produced more than a concentration of
10^6 cells/ml in stationary cultures (5ml). In a spinner flask culture of
500ml, MaBr-93 grew to 1x10^6 cells/ml from 2x10^5 cells/ml. In a jar
fermenter culture of 40 liters, it grew to 6.5x10^5 cells/ml from 1.5x10^5
cells/ml.
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