| Publication number |
CLPUB00527 |
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| Authors |
Manaka K.-I., Nonaka N., Yamada T., Hirai H. |
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| Title |
Monoclonal antibodies against large-cell carcinoma of the lung. |
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| Citation |
Dokkyo J. Med. Sci. 12:31-41(1985) |
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| Web pages |
https://dl.ndl.go.jp/info:ndljp/pid/11023962 |
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| Abstract |
Monoclonal antibodies (MoAbs) against a newly established cell line (HLC-2)
derived from a human large-cell lung carcinoma were prepared by
hybridization between the murine myeloma P3U1 and spleens from immune
BALB/c mice. The specificity of the MoAbs was screened with the panels of
non-neoplastic or neoplastic cultured cells and the tissues of the same
cancer patient as immunogen donor by using antibody-dilution curves and
histological preparations of avidin-biotin-conjugated-peroxidase antibody
technique. Several MoAbs reacted against immunogen cultured cells (HLC-2)
and autologous tumor tissues. Among those MoAbs, two MoAbs (1A7 and 6B3)
reacted at very low or undetectable levels with non-neoplastic cells
(primary-cultured lung epithelial cells and the autologous lung tissues).
In the lung carcinomas, MoAb 1A7 did not react against QG-90 cells (small-
cell carcinoma), PC-13 (large-cell carcinoma) and SK-MES-1 cells (squamous
cell carcinoma), but reacted against Luci 10 cells (adenocarcinoma). And
MoAb 6B3 reacted only against SK-MES-1 cells and HLC-2 cells. Both MoAbs
did not react against other types of tumor cell line (HeLa, uterus
carcinoma and HuL-1, hepatoma). That is, the epitope recognized by MoAb
1A7 exists possibly associating with adenocarcinoma and normal glandular
epithelium of the lung. That of MoAb 6B3 is suggested to associate with
squamous-cell carcinoma and large-cell carcinoma and to be specific in
neoplastic cells of the lung. The monoclonal antibody-dependent macrophage-
mediated cytotoxicity assay was performed. The inhibition activities of
MoAbs to the target cell growth were found to be enhanced by mixed
cultures with macrophages. And MoAbs 1A7 and 6B3 showed a strong inhibitory
effect. All these findings suggest the potential of the identification of
the molecules of lung cancer-associated antigens and the application on
the clinics.
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| Cell lines |
CVCL_WZ40; 6B3 [Mouse hybridoma against human LGALS3BP] CVCL_WZ42; HLC-2 |
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