| Publication number |
CLPUB00523 |
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| Authors |
Ye K.-L., Ren S.-L., Zhou L.-D., Lin Y.-Z., Xu Z.-Y., Su D.-L., Yu Y.-F., Ma Z.-C. |
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| Title |
Preliminary observation on some biological characteristics of human hepatoma cell line YY-8103. |
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| Citation |
Shanghai Yi Ke Da Xue Xue Bao 11:423-429(1984) |
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| Web pages |
https://en.cnki.com.cn/Article_en/CJFDTOTAL-SHYK198406006.htm |
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| Abstract |
A human primary liver carcinoma cell line has been established in vitro in
1981, which was designated as YY-8103. Specimen of the primary liver
carcinoma was obtained from a 60-year old male patient at operation. This
cell line has been transferred through more than 130 passages. The
characteristics of the cell line are as follows: Morphologically, the
cells are polygonal epithelial-like. The nuclei are large in size and
irregular in shape, and mitosis can be seen under EM. The mode of
chromsome numbers of the cell line was 64. Heterotransplantation was
successfully achieved in nude mice, in which the features of the
histological section were in accordance with those of the original
specimens. The tumor nodules of nude mice could be retransplanted and
recultured in vitro. An anomalous pattern of LDH isozyme for the cell line
was found with the highest activity of LDH-3. No HBsAg was detected in the
culture medium nor in the homogenized cell but AFP was positive in sera of
nude mice with growing tumor nodules. The suspension of the cell could be
agglutinated by Con-A. The cell line is susceptible to measles virus and
herpes simplex virus, Therefore, the YY-8103 cell line may be used in the
basic investigation of liver carcinoma and virus culture.
|
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| Cell lines |
CVCL_WY40; YY-8103 |
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