| Publication number |
CLPUB00521 |
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| Authors |
Freed J.J., Howard S., Lerro A.V., Dodson L.A., Sesok D., Knudson A.G. Jr. |
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| Title |
Hereditary renal tumors in the rat: cell lines from adenocarcinomas induced by the Eker mutation. |
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| Citation |
Proc. Am. Assoc. Cancer Res. 31:317-317(1990) |
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| Abstract |
The Eker mutation is an autosomal dominant: in the rat that induces renal
adcnocarcinomas among heterozygotes; homozygotes die before birth. It thus
provides an animal model for a putative anti-oncogene. We have established
a panel of 20 cell lines, each derived from an independent renal tubular
adenocarcinorna arising in heterozygotes. Tumor tissue was cultured on
collagen surfaces in a hormone-supplemented, serum-free medium. An initial
outgrowth of thinly spread, apparently epithelial, often multinucleate,
cells gave rise to foci of densely packing cells that were further
propagated as established epithelial cell lines. These cell lines have
been authenticated as to rat origin by cytogenctic analysis, are
epithelial by ultrastructure and immunostaining for cytokeratins and
multiply slowly in soft agarose. Most lines tested arc non-tumorigenic in
nude or scid mutant mice but exceptional lines have produced tubular
carcinomas after implantation into the testes of scid mice. Cytogenetic
analysis shows both numerical and structural karyotypic abnormalities in
all the lines.
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| Cell lines |
View cell lines |
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