| Abstract |
Fibroblasts from patients with Nijmegen breakage syndrome (MBS) were
transformed by infection of SV40 virus. The cells used were 0816J, 0823H,
1129W, 1022Q, 1217G (each Czech), GM07166 (USA), 2239, 2240 (each German).
Although all virus-infected fibroblasts showed the prolonged life span,
only GM07166SV clone was immortalized. Southern blot analysis indicated
the SV40 virus genome present at this clone. The GM07166SV clone was
high sensitive to ionizing radiation-induced cell killing, similar to
parental GM07166 cells. The hybrid clones obtained between GM07166SV(gpt)
and AT5BIVA(neo) showed the restoration of radiation sensitivity, implying
the different mutations of both diseases. We are also trying to determine
the chromosome carrying NBS gene, by introduction of a single chromosome
via micro-cell fusion technique.
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