| Publication number |
CLPUB00495 |
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| Authors |
Cavalloro R., Scovassi A.I., Bertazzoni U. |
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| Title |
Aspects of DNA synthesis in cells and embryos of med-fly (Ceratitis capitata Wied.). |
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| Citation |
(In) Fruit flies of economic importance; Cavalloro R. (eds.); pp.212-218; A.A.Balkema Publishers; Rotterdam (1983) |
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| Web pages |
https://nucleus.iaea.org/sites/naipc/twd/Lists/Symposia/Attachments/1/1st-ISFFEI-1982-Athens-Greece.pdf |
|---|
| Abstract |
We have analyzed the DNA polymerase activities in cultured cells and
embryos of Ceratitis capitata Wied. and we have observed that the three
eukaryotic polymerases-alpha, -beta and - gamma were detectable in crude
extracts. However the activity responding to polymerase-beta did not
correspond to a true DNA polymerase-beta upon subsequent sedimentation and
purification procedures. Our studies with aphidicolin, the specific
inhibitor of polymerase-alpha, indicate that cultured Med-fly cells show
an arrest in cellular growth at much higher concentration of the drug than
that observed for other eukaryotic cells. This is reflected also by a
decreased sensitivity to aphidicolin of DNA polymerase-alpha purified from
Med-fly embryos.
|
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| Cell lines |
CVCL_Z884; CEC CC 130 |
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