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Cellosaurus publication CLPUB00493

Publication number CLPUB00493
Authors Eppler A., Adam G., Sander E.
Title Nutritional requirements and growth of two cell-lines from the aphid parasite, Ephedrus plagiator under different culture conditions.
Citation (In book chapter) Invertebrate systems in vitro; Kurstak E., Maramorosch K., Dubendorfer A. (eds.); pp.59-62; Elsevier; Oxford; United Kingdom (1980)
Abstract For the investigation of virus-vector relationships on a cellular level, cell lines of vectors are a useful tool (Black 1969). Aphids are the most prominent group of insect vectors of plant viruses and cell lines of aphids would be a useful tool. However, no aphid cell lines are as yet available (Valenta 1977). While dealing with primary cultures of the Damson hop aphid Phorodon humuli Schrk. Two cell lines of the insect Ephedrus plagiator (Aphidiidae, Hymenoptera), a parasite of the aphid were established in 1976. The cell lines were derived from larval tissue. Since parasite and aphid embryos develop under comparable conditions, the new cell lines were tried as a tool to optimize media and culture conditions for aphid cells. Several media developed for aphid cell culture, an insect cell line medium and an artificial medium were tested.
Cell lines CVCL_WK70; IBT-EP 1
CVCL_WK36; IBT-EP 2