| Abstract |
Human papillomavirus (HPV) types 16, 18, and 33 play a causative role in
the development of uterine cervix cancer. Fourteen cell lines derived from
human genital organs are maintained at the Institute for Fermentation,
Osaka (IFO). To know whether these cell lines were infected by HPV types
16, 18, and 33, we tried to detect these viral DNAs by polymcrase chain
reaction (PCR) with specific primers homologous to the E6 open reading
frame. All seven cell lines derived from uterine cervix carcinoma
possessed the HPV types 16 or 18 DNA, whereas none of seven cell lines
derived from uterus body cancer or ovarian cancer possessed the HPV types
16, 18, and 33 DNA. In addition, this PCR was applicable to detect the
cross-contamination of cell lines by HeLa cells.
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