| Abstract |
Ten clones were picked from cultured colonies of CaEs-17 cell line and
developed to a subcell line. The values of SF2 were measured for each
subcell line. Two radioresistant subcell lines, clone 7 and clone 10 were
established. According to survival curve assay, for wild type, the value
of D0 was 1.57 Gy, DQ was 1.07 Gy, N was 1.96, SF2 was 0.41. For clone 7,
the value of D0 was 1.64 Gy, DQ was 1.85 Gy, N was 3.07, SF2 was 0.53. For
clone 10, the value of D0 was 1.58 Gy, DQ was 2.04 Gy, N was 3.63, SF2 was
0.61. Clone 7 and clone 10 have much higher values of DQ and N than those
of wild type. There was amplification of H-ras gene in clone 10 after 2 Gy
irradiation. The amplification of H-ras gene in clone 10 after 2 Gy
irradiation might be involved in heterogeneity of CaEs-17 cell line.
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