| Abstract |
The aim of this work is to obtain transplantable carp lines (Cyprius
carpio) and Siberian sturgeon (Acipenser baeri) and to assess the
possibility of their use for virological studies of fish. To achieve this
goal, it was necessary to solve the following tasks: to explant and
maintain for a long time the cells of the ovarian tissue of carp,
parenchymal organs and fins of Siberian sturgeon in their primary culture
for use as a starting material in the production of transfected cell lines.
We establishd a transplantable line of ovary cells of immature carp,
transplantable cell lines of a pool of parenchymal organs and fins of
Siberian sturgeon, we determined the sensitivity of the resulting cell
lines to widespread fish viruses of different taxonomic groups, we carred
out the certification of the obtained cell lines and deposited them in the
Russian collection of cell cultures.
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