| Publication number |
CLPUB00442 |
|---|
| Authors |
Khurad A.M., Raina S.K., Pandharipande T.N. |
|---|
| Title |
Establishment of pupal fat body cell line from Mythimna convecta and its susceptibility to Nosema locustae (Microsporida). |
|---|
| Citation |
(In book chapter) Proceedings of the VIII international conference on invertebrate and fish tissue culture; Fraser M.J. Jr. (eds.); pp.26-35; Tissue Culture Association; Anaheim; USA (1991) |
|---|
| Abstract |
A continuous cell line designated as BPMNU-MyCo-1 has been derived from
the fat body of maize armyworm, Mythimna convecta. Primary cultures were
initiated in MGM-448 medium supplemented with 5% FBS and 3% Bombyx mori
serum and subsequently adapted to MM medium with 3% FBS. The cell
population consisted predominantly of small and large round suspended
cells with few spindle shaped ones. The karyotype was typical of
lepidopterans consisting of 80-100 microchromosomes. Cells could grow at a
wide range of temperatures from 20-30 C in MGM-448 medium, however, they
were sensitive to changes in pH of the medium. The population doubling
time of the cell line was 40 hrs at 25 C, and it could be stored at 4 C
for five months. The cell line was inoculated with Nosema locustae spores
and infection appeared three days postinoculation. By the seventh day some
cells were filled with spores. At 15 days postinoculation 32% of the cells
were infected with spores with a total yield ranging from 2.2x10^6 to
4.0x10^6 /ml. Infected cells were subcultured and by the fourth passage
spore production decreased. The harvested spores were infectious to
Locusta migratoria.
|
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| Cell lines |
CVCL_AV10; BPMNU-MyCo-1 |
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