| Publication number |
CLPUB00434 |
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| Authors |
Ishiwata I., Nozawa S., Kiguchi K., Kurihara S., Okumura H. |
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| Title |
Establishment of human uterine cervical cancer cell line and comparative studies between normal and malignant uterine cervical cells in vitro. |
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| Citation |
Nihon Sanka Fujinka Gakkai Zasshi 30:731-738(1978) |
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| Web pages |
https://ci.nii.ac.jp/naid/110002117678/en |
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| Abstract |
The cell lines designated SKG-2 and SKG-2B were established from human
uterine cervical cancer (epidermoid carcinoma) before and after bleomycin
treatment. The SKG-2B cell line was subcultivated more than 45 times
within 14 months. The cultured cells appeared epithelial in shape, with a
pavement-like arrangement and grew in multi-layers without contact
inhibition. The cytology revealed anaplastic and pleomorphic features. In
electron microscopy, the cells were characterized by smooth nuclear
membranes, multiple large nucleoli, numerous microvilli, desmosomal cell
contacts and few tonofibrils. The chromosome studie showed aneuploidy and
the modal chromosomal number was stable in the hypo-diploid range. Thc
marker, a large submetacentric chromosome, was constantly present. The SKG-2B
cells were transplanted subcutaneously to nude mice and submucousally to
immunedepressed hamster cheek pouches, and produced tumor which resembled
the original tumor. No differences between SKG-2 and SKG-2B could be
identified in the growth and morphological characteristics. On the contrary,
the normal uterine cervical squamous epithelial cells in vitro were uniform
in shape and growth, and showed a limited life span. The cells showed a
favorable outgrowth from the explant and cornification in the growing
process. The cells could not be subcultivated and eventually degenerated
after maintaining the culture for 2 months.
|
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| Cell lines |
CVCL_2794; SKG-II CVCL_VI91; SKG-IIB |
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