| Publication number |
CLPUB00433 |
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| Authors |
Morier Diaz L., Lopez-Santa Cruz D.I., Alvarez C.M., Lorenzo Y.C., Llanes D.M. |
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| Title |
Obtainment of CLA-HT cell subline and study of its susceptibility for the isolation and multiplication of the dengue virus. |
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| Citation |
Rev. Cubana Med. Trop. 66:424-432(2014) |
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| Web pages |
https://www.medigraphic.com/cgi-bin/new/resumenI.cgi?IDARTICULO=56243https://www.researchgate.net/publication/287745047http://scielo.sld.cu/scielo.php?pid=S0375-07602014000300010&script=sci_abstract |
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| Abstract |
Introduction: The identification of viral serotypes is an important issue
for dengue diagnosis. Despite the existence of other identification
methods, the viral isolation in cell cultures and determination by
immunofluorescent technique remain as the most used. Therefore the search
for more sensitive cellular systems has been a repeated topic during many
years for dengue virus and other agents. Objective: to obtain a cell
subline from CLA-1 clone (Aedes pseudoscutellaris), that grows at 28
Celsius, capable of multiplying at 33 Celsius (CLA-HT) and to evaluate its
usefulness for Dengue virus isolation and identification.
Methods: By using a CLA-1 clone of the AP-61 (Aedes pseudoscutellaris)
cell line, it was possible to obtain a strain or cell subline CLA-HT
capable of growing at 33 oC (CLA-HT) through a temperature selection
method. Its sensitivity for the multiplication of the 4 dengue virus
serotypes and its efficiency for direct virus isolation from acutely ill
patients' sera were studied in comparison to C6/36 HT cell line (A.
albopictus).
Results: Four dengue virus serotypes grew in CLA-HT cell subline but some
serotypes were detected later in CLA-HT than in C6/36 HT. For dengue
isolation from serum samples taken in acutely ill patients, the CLA-HT
subline detected 40% positive samples whereas C6/36 HT did 50%.
Discussion: CLA-HT is able to detect all dengue virus serotypes from 96
hours on post inoculation. It makes the new cell line useful for research
as an alternative system and the direct isolation efficiency is good to be
applied in large outbreaks. The most important advantage of CLA-HT is the
possibility of giving rapid answer in emergency situations in low resource
laboratories since it does not require special incubation conditions with
CO2.
Conclusions: A CLA-HT subline was obtained from CLA-1 line and it grows at
33 Celsius and capable of detecting 4 dengue virus serotypes. Its
isolation efficiency is slightly lower than that of C6/36 HT subline, but
it may be used as an alternative system for dengue virus isolation, mainly
in resource-poor laboratories that do not have the optimal conditions for
incubation.
|
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| Cell lines |
CVCL_VH38; CLA-HT |
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