| Publication number |
CLPUB00408 |
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| Authors |
Zach S., Grun J., Bauer A.T., Pilarsky C., Grutzmann R., Weng H., Dooley S., Wilhelm T.J., Gaiser T., Ruckert F. |
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| Title |
CCC-5, a new primary cholangiocellular cell line. |
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| Citation |
Int. J. Clin. Exp. Pathol. 10:2451-2460(2017) |
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| Web pages |
https://www.ijcep.com/files/ijcep0022410.pdf |
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| Abstract |
Background: Cholangiocellular carcinoma (CCC) is a rare tumor entity and
the availability of systemic treatment is limited. Better knowledge of the
tumor physiology might lead to an improved treatment. However, basic
research is dependent on the availability of cell lines. As there are only
few CCC cell lines disposable, we aimed to isolate new CCC cell lines from
surgical samples.
Material and methods: The cell line was established from a 59-year-old
Caucasian male patient who suffered from malignant pleural effusion of a
distal cholangiocellular carcinoma (CCC). The cell line was established by
explant culture and further characterized with immunohistochemistry and
Western blotting. Mutational analysis was performed by means of AmpliSeq
Cancer Panel v2. Additionally, we tested sensitivity towards commonly used
chemotherapeutics.
Results: Immunohistochemistry and Western blotting showed expression of
tumor markers typical for CCC cell lines. Analysis of sensitivity towards
chemotherapeutics revealed that Irinotecan was the only agent with
significant effect on cell proliferation. Mutational analysis identified
different mutations in important oncogenes and tumor suppressor genes.
Conclusions: The new cell line displays the typical characteristics of a
CCC cell line. This new cell line might help to add new insights into this
tumor entity and thereby help to find better treatment options for
patients with CCC.
|
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| Cell lines |
CVCL_LM83; CCC-5 |
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