| Publication number |
CLPUB00401 |
|---|
| Authors |
Arifin M.A., Mel M., Raus R.A., Sharifah S.H., Ideris A. |
|---|
| Title |
Comparison of growth rate and viability of DF-1 cell line in different culture media. |
|---|
| Citation |
(In conference) Abstracts of the 2nd international conference on science and technology (ICSTIE 2008); pp.1-5; Universiti Teknologi MARA; Pulau Pinang; Malaysia (2008) |
|---|
| Web pages |
http://irep.iium.edu.my/id/eprint/29284 |
|---|
| Abstract |
One of the commercial cell line that gained attention for the study of
viral vaccine was an immortalized cell line derived from chicken embryo
fibroblast (CEF) cells. Unlike CEF cells, DF-1 cells have higher
proliferation rate and also can be subcultured infinite number of times.
In this study, the growth profile of DF-1 cell line was investigated by
undergoing series of optimization process by testing it out on several
culture media and also by manipulating the components of culture media
which were sodium bicarbonate and serum. At the end of this study it has
been figured out that DF-1 achieved the highest cell culture density in
Dulbecco's modification of Eagle's medium (DMEM) and with 1.24 g/L of
NaHCO3 and 5.5% serum, it yielded the shortest doubling time.
|
|---|
| Cell lines |
CVCL_0570; UMNSAH/DF-1 |
|---|
