| Publication number |
CLPUB00384 |
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| Authors |
Furukawa M., Umeda R., Hatano M. |
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| Title |
Transfer of EBV (Epstein-Barr virus) genome from lymphoblastoid cells to established epithelial cells derived from human tissues Experimental studies on the viral etiology of nasopharyngeal carcinoma (NPC) related to EBV infection. |
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| Citation |
Kanazawa Daigaku Juzen Igakkai Zasshi 84:190-210(1975) |
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| Web pages |
https://kanazawa-u.repo.nii.ac.jp/?action=repository_uri&item_id=19014&file_id=26&file_no=1https://ci.nii.ac.jp/naid/110004084862/en |
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| Abstract |
In order to investigate an etiological role of Epstein-Barr virus (EBV) in
Nasopharyngeal carcinoma (NPC), a transfer of EBV-genome to cultured
epithelial cells derived from nasopharynx was attempted here. This
transfer or infection of EBV is usually detected by immunofluorescent
staining of EB viral capsid antigen (EB-VCA) or by more sensitive staining
of EBNA (EBV-associated nuclear antigen) in EBV-infected cells. 24 sera
from NPC or NPS (nasopharyngeal sarcoma) patients were screened for the
EBNA staining by ACIF (anti-complement immunofluorescence) test, using a
frozen section material from a NPC patient and 16 cultured cell lines. 3
sera (NPC: 2 and NPS: 1) were, thus, selected for the experimental EBV
infection. These 3 sera were determined to be useful from their positive
EBNA stainings for NPC-tissues and EBV-carrying human lymphoblastoid cells
(HLBC), or their negative ones for human epithelial cells and PHA
(phytohemagglutinin)-transformed HLBC. Other 2 NPC sera of 24 sera gave
either nuclear or cytoplasmic staining 10 of human epithelial cell lines
except 2 non-human cell lines (VERO and THEL cells). This unique staining
by 2 NPC sera appeared to be heterophilic rather than EBV-specific. Its
heterophilic properties were also suggested by a precipitation test in
agar between these sera and histone fraction.
|
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| Cell lines |
CVCL_IY39; 2-27-Ad |
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