| Publication number |
CLPUB00379 |
|---|
| Authors |
Yang Y.-Q., Tao H.-H., Yang H.-C. |
|---|
| Title |
Establishment of human primary gallbladder cell line SGC-996. |
|---|
| Citation |
Tong Ji Da Xue Xue Bao (Yi Xue Ban) 24:457-459(2003) |
|---|
| Web pages |
https://en.cnki.com.cn/Article_en/CJFDTOTAL-TJIY200306000.htm |
|---|
| Abstract |
Objective: To establish human primary gallbladder cancer cell line SGC-996
and observe its cytobiological characteristics.
Methods: The gallbladder cancer cells were isolated from the primary
mastoid adenocarcinoma of the gallbladder obtained from a 61 year-old
female patient. The cancer cells were cultured in Eagle and RPMI 1640
media with fetal bovine serum.
Results: This cell line could grow steadily in RPMI1640 with 10% fetal
bovine serum and proliferate rapidly. The cells were epithelial-like and
had large nuclei with one or more nucleoli. The cells could form multi-
layered colonies. Doubling time of cell proliferation was about 48 h.
Mitotic index was high. Chromosome frequency distribution belonged to
hypotriploidy. All athymic rude mice could form the transplant tumors in
the test of subcutaneous inoculation. A plentiful of microvilli and fewer
cell junctions such as desmosomes were observed by transmission electron
microscopy. Virus granule, mycoplasma and bacteria were negative.
Conclusions: SGC-996 cells accorded with the general characteristics of the
cell line in vitro.
|
|---|
| Cell lines |
CVCL_M737; SGC-996 |
|---|
