| Publication number |
CLPUB00373 |
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| Authors |
Shimoda T., Kuramoto H., Morisawa T., Tsunoda S., Shiotsu H., Arai M. |
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| Title |
A new cell line (HCSC-1) established from small cell carcinoma of the uterine cervix. |
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| Citation |
Nihon Sanka Fujinka Gakkai Zasshi 40:1325-1325(1988) |
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| Web pages |
https://ci.nii.ac.jp/naid/110002226658/en |
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| Abstract |
A new cell-line (HCSC-1) was established from the ascites of the patient
with small cell carcinoma of the uterine cervix. The results were follows.
1) From the growth curve of HCSC-1 population doubling time was 24.3
hours (4th culture) and plating efliciency was 1.9%. 2) Under phase-
contrast microscope, cancer cells were observed to grow as colony like
cell aggregates and to attach each other with piling up. B) From the view
of the Pap. stain, small cells with markedly increasing N/C ratio
aggregated each other. The nuclear chromatin was coarsely granular but
the nuclear rim was not so thick. The nucleoli were relatively prominent
and they had a tendency to multiplicity, 4) A fairly large number of
cells showed argyrophilia by the Grimelius stain, and neurosecretory
granules in cytoplasm were observed on electron microscopic study. 5) The
secretion of NSE and CEA in culture media chronologically increased, and
was enhanced by low concentration (0.1 mM) of dibutyryl cyclic AMP. 6)
The tumor of the nude mice after transplantation showed small cell
carcinoma like the original cancer. 7) The peak of the chromosome number
was at hyper-diploid range and modal number was 52. 8) This rare cell-
line established was shown as a useful experimental model in vitro.
|
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| Cell lines |
CVCL_2919; HCSC-1 |
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