| Abstract |
Cell culture of insects is one of the most important techniques for the
basic research of life science and biotechnology. Since Grace first
established the insect continuous cell line, one fourth century has passed.
During this period, many culture media were developed and more than 51
cell lines of Lepidoptera were established; thus the cell culture became a
mature technique in the field of establishment of continuous cell lines.
The application of insect cell culture is expected for the virus
production in vitro aiming at the pest control, and for the production of
useful proteins by the Baculovirus cell line system. For the economic
production of viruses or useful proteins, several problems, such as 1)
establishment of highly proliferative cell line, 2) selection of cell
highly susceptible to viruses, 3) development of low-cost medium, and 4)
large-scale culture of cells, must be solved. In the present paper, I
report the establishment of continuous cell lines of the silkworm, Bombyx
mori, and its usefulness for the research on the relationship between the
multiplication of Bombyx mori nuclear polyhedrosis virus and the culture
condition of cells. In addition, the cell line of the cabbage armyworm,
Mamestra brassicae, was also established.
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