| Abstract |
The transplantable tumor animal model may objectively simulate the process
of growTH in human tumor. Accordingly, it was generally applied to the
research of the tumorigenic mechanism and the screening of the anti-tumor
drugs. In the long process of passage, tumor cell strains are exposed to
variety of unstable factors, such as genetic pollution and genovariation.
Therefore, through a long time it is possible that variation of genetic
mass and the following changes in biological characteristics may decrease
the homogenicity and comparison of tumor animal models. If wanting to keep
the stability of the animal models of transplanted tumors, we have to give
quality control on the tumor cell strains. Hepatoma 22 (H22) is one of the
most usually used mice transplanted tumor cell lines and widely applied to
make the mouse tumor models. This cell line has been set up for nearly 50
years. Many scholars reported that it had already undergone biggish
genetic variance and the cell strains conserved in different institutes
had been found large difference. Liu junxu etc have made H22 cloning and
got 4 clone strains, including H22-H8D8, H22-H2C4, H22-H2G4 and H22-H2E10.
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