| Publication number |
CLPUB00366 |
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| Authors |
Fan T.-J., Guo X.-Y., Jiang G.-J., Xu X.-H., Sun A., Xu B. |
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| Title |
Establishment of a continuous gill cell line from spotted halibut, Verasper variegatu. |
|---|
| Citation |
Per. Ocean Univ. China 40:69-74(2010) |
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| Web pages |
https://en.cnki.com.cn/Article_en/CJFDTotal-QDHY201009011.htm |
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| Abstract |
To establish a novel cell line from spotted halibut, Verasper variegatus,
primary culture of spotted halibut gill tissue was initiated with methods
of trypsin digestion, and the cells were successfully sub-cultured through
optimizing the medium formulation and culture conditions. The gill cells
grew well at 22 Celsius in DMEM/F-12 medium (pH 7.2) which contained 2%
fetal bovine serum (FBS), N-acetyl-D-glucosamine, carboxymethyl-
chitooligosaccharide, basic fibroblast growth factor (bFGF) and insulin-
like growth factor-I (IGF-I). The cells showed fibroblastic shape, grew
rapidly and formed a confluent monolayer after 30 days of cultivation. The
gill cells could be sub-cultured subsequently and maintained their
original growth rate after cryopreservation and resuscitation. The
population doubling time of the cell line was 43.6 hours at passage 60.
Chromosome analysis showed that some cells were chromosomal aneuploidy but
most of them had a modal chromosome number of 46, which had a normal
diploid karyotype of 23 pairs of telocentric chromosomes at passage 60. A
continuous spotted halibut gill cell line has been established which is
being sub-cultured to passage 76 now. The establishment of this cell line
will make great contribution to the study of mechanisms of cell-virus
interactions and the development of viral vaccine for fish viral disease
control.
|
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| Cell lines |
CVCL_IN54; SHG [Verasper] |
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