| Abstract |
A stable cell line, BSBS (black seabream spleen), was established from the
cells in spleen of black seabream, Acanthopagrus schlegeli, and
characterized. Subculture maintained more than 60 passages and
morphologically, BSBS cell was epithelioid cell. The cells grew optimally
at 20 Celsius in Leibovitz's L-15 medium supplemented with 10% fetal
bovine serum with incubation temperature of 20 Celsius. BSBS cells
supported the growth of marine birnavirus (MABV Y-6), chum salmon reovirus
(CSV), spring viremia of carp virus (SVCV) and hirame rhabdovirus (HIRRV).
Thus, the new cell line may be useful for studying wide range of fish
viruses.
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