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Cellosaurus publication CLPUB00355

Publication number CLPUB00355
Authors Franca C.M., Oliveira P.T., Jaeger M.M.M., Jaeger R.G.
Title Salivary gland tumors: a cytodifferentiation in vitro study.
Citation J. Dent. Res. 76:1001.287-1001.287(1997)
Abstract Adenoid cystic carcinoma (cylindroma) and pleomorphic adenoma are neoplasms derived from the intercalar duct of salivary glands. We studied differentiation patterns of adenoid cystic carcinoma and pleomorphic adenoma, by comparing cell cultures from these two neoplasms. The first differentiation parameter assessed was the presence of cytoskeletal proteins. Immunofluorescence showed that pleomorphic adenoma cells (AP2) showed only vimentin, while cylindroma cells (CAC2) expressed vimentin, muscle-specific actin and the cytokeratins 8, 14, 18 and 19. This preliminary result suggested that CAC2 cells would be more differentiated than AP2 cells. To further investigate this result, and by knowing that basement membrane proteins induce citodifferentiation, we cultured both cell lines on the top of a reconstituted basement membrane (Matrigel). In these conditions, AP2 cells were not able to differentiate into ductiform structures, while CAC2 cells morpho and cytodifferentiated into authentic ductiform structures, as demonstrated by fluorescence confocal microscopy. Knowing that AP2 cells originate ductiform structures only when grown inside a three-dimensional environment of Matrigel, we may infer that CAC2 need a less complex induction to form the same structure, Thus, we suggest that cylindroma cells are derived from intercalar duct cells in a more advanced stage of differentiation than the cells that originate pleomorphic adenoma.
Cell lines CVCL_6883; CAC2