| Abstract |
Objective: To isolate S180 stored in Beijing Cancer Institute and our
department by the single cell cloning technique and to study property of
those clonal cells.
Methods: SDS polyacrylamide gel electrophoresis, scanning electron
microscopy and immunohistochemical method were used.
Results: Five sub-lines (S2H7, S1F11, S2D7, S2D9, S1B11) were isolated
from S180 stored in Beijing Cancer Institute, three sub-lines (S1H10,
S3F5, S1E11) were isolated from S180 stored in our department. Ascites of
three sub-lines S1H10, S3F5 and S2H7 were bloody; S1F11 was tiny bloody;
S1E11, S2D7, S2D9, S1B11 were not bloody property in 7 days of
intraperitoneal inoculation. As for surficial structure under scanning
electron microscope S3F5 had plentiful microvilli; S1B11 surface had a lot
of prominences; S1F11 surficial microvilli were fewer than S3F5; S2D9 and
S2H7 had different many tiny crests and tiny folds; S2D7 was more smooth.
DNA contents were 8.5, 9.3, 7.9, 8.5, 8.6, 8.3 pg for six sublines S1B11,
S2D7, S3F5, S2H7, S2D9, S1F11 respectively by flow cytometry. With
immunohistochemistry the different clonal cells had different reactions
with 11 kinds of antibodies (once reaction positive, positive rate was
more than 97%). S1H10 and S2D9 were detected by SDS-PAGE, with 33 and 30
protein bands respectively.
Conclusions: Eight sub-lines showed different properties.
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