| Publication number |
CLPUB00331 |
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| Authors |
Basiri E., Ardehali S.M., Doroudchi M., Mahboudi F., Kharazmi A., Ghaderi A. |
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| Title |
Production and characterization of murine monoclonal antibodies to Leishmania Gp63 antigen. |
|---|
| Citation |
Iran. J. Immunol. 1:194-199(2004) |
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| Web pages |
https://iji.sums.ac.ir/article_16815.html |
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| Abstract |
Background: Production of monoclonal antibodies to Leishmania antigens
assists the identification and characterization of these organisms.
Objective: Production of monoclonal antibodies against epitopes on the
gp63.
Methods: Two murine monoclonal antibodies to gp63 were produced and
characterized. The reactions of both antibodies with soluble leishmanial
antigens, purified gp63 and truncated recombinant gp63 molecules were
studied by an ELISA assay. These two antibodies reacted with the crude
soluble antigens prepared from 4 reference strains of Leishmania, 10
isolates from the patients, purified gp63 and recombinant gp63 molecules.
However, no reaction with several non-leishmanial antigens was observed.
Reaction of both antibodies with the intact recombinant gp63 and truncated
molecules were compared.
Results: The results indicated that the two antibodies specifically
recognize two different epitopes on the gp63 molecule.
Conclusions: Possible applications of such antibodies in searching for
immunogenic epitopes are discussed.
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| Cell lines |
CVCL_CW40; 4C8 [Mouse hybridoma against L.donovani gp63] CVCL_CW39; 5C5 [Mouse hybridoma against L.donovani gp63] |
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