| Publication number |
CLPUB00330 |
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| Authors |
Basiri E., Ardehali S.M., Kamali-Sarvestani E., Habibagahi M., Sharifi I., Ghaderi A. |
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| Title |
Production and partial characterization of monoclonal antibodies to Leishmania. |
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| Citation |
Iran. J. Med. Sci. 27:75-79(2002) |
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| Web pages |
https://ijms.sums.ac.ir/article_40252.html |
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| Abstract |
Background: Monoclonal antibody technology allows identification of
amastigote-specific antigen in human tissue biopsies and search for a
suitable vaccine candidate, which may induce a long lasting immunity.
Objectives: Production and partial characterization of seven monoclonal
antibodies against Leishmania tropica promastigotes.
Methods: Seven murine monoclonal antibodies of the IgG isotype were
produced against the stationary stage promastigotes of Leishmania tropica.
Results: Extensive cross reactivity was found within 4 reference strains
and isolates from the patients. Non-Leishmanial antigens were not
recognized by any of the antibodies. One antibody recognized a diffuse
band 64 to 86 kDa, and the other 6 recognized 1, 2, or more bands in
immunoblotting of soluble antigens of promastigotes. The reaction of these
7 antibodies with the soluble extracts of 4 reference strains of
Leishmania and 10 isolates from patients was studied.
Conclusions: Although these antibodies were not suitable for identification
purposes, the characterization of shared epitopes may be considered in
studying the antigenic structure of these organisms. Further experiments
may confirm the idea that some of these epitopes are suitable vaccine
candidates.
|
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| Cell lines |
CVCL_CW38; 9H7 |
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