| Publication number |
CLPUB00323 |
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| Authors |
Swaminathan T.R., Gopalakrishnan A., Basheer V.S., Divya P.R. |
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| Title |
Development and characterization of a new fish cell line from honeycomb grouper, Epinephelus merra, Bloch 1793. |
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| Citation |
Indian J. Anim. Sci. 82:1100-1105(2012) |
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| Web pages |
http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/23682 |
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| Abstract |
A new cell line HGF was developed from fin tissue of Epinephelus merra
Bloch, 1793. The cell line was maintained in Leibovitz's L-15 supplemented
with 15% FBS and cells have been subcultured 45 times. The HGF cell line
consists predominantly of fibroblastic-like cells. The cells were able to
grow at temperatures between 25 Celsius and 32 Celsius with optimum
temperature of 28 Celsius. The growth rate of fin cells increased as the
FBS proportion increased from 2 to 20% at 28 Celsius with optimum growth
at the concentrations of 15 or 20% FBS. After confluence, the cells were
sub-cultured with a split ratio of 1:2. The cells showed fibroblastic-like
morphology and reached confluence on the fourth day after subculture.
Polymerase chain reaction amplification of mitochondrial 16S rRNA and COI
indicated identity of these cell lines with those reported from this
animal species, confirming that the cell lines were of honeycomb grouper
origin. The cells were successfully cryopreserved and revived at passage
numbers 10, 20 and 30. The bacterial extracellular products from Vibrio
cholerae MTCC 3904 was found toxic to this cell line.
|
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| Cell lines |
CVCL_6E26; HGF [Epinephelus] |
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