| Publication number |
CLPUB00316 |
|---|
| Authors |
Sokolovskaya A.A., Zabotina T.N., Blokhin D.Y., Inshakov A.N., Mikhailov A.D., Kadagidze Z.G., Baryshnikov A.Y. |
|---|
| Title |
CD95-deficient cells of Jurkat/A4 subline are resistant to drug-induced apoptosis. |
|---|
| Citation |
Exp. Oncol. 23:175-180(2001) |
|---|
| Web pages |
https://istina.ips.ac.ru/publications/article/434992576/ |
|---|
| Abstract |
We developed unique CD95-deficient subline (Jurkat/A4) from the parent
Jurkat/WT cell line by continuous culture in medium containing apoptosis-
inducing anti-CD95 MAb, clone IPO-4 (IgM). In contrast to the parental
Jurkat/WT line, which displays relatively high expression of CD95 (60-80%
antigen-positive cells), cell-surface expression of the CD95 was entirely
negative in the Jurkat/A4 cell line (only 4% of antigen-positive cells).
The Jurkat/A4 cells are resistant to induction of apoptosis by both
anti-CD95 mAb IPO-4 and chemotherapeutic drugs, whereas the wild type clone
Jurkat/WT undergoes time-dependent apoptosis. Thus, these results suggest
the possible participation of CD95 pathway in the development of
drug-induced apoptosis.
|
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| Cell lines |
CVCL_AR84; Jurkat/A4 |
|---|
