| Publication number |
CLPUB00313 |
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| Authors |
Jeon K.-L., Cho S.-B., Hwang K.-K. |
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| Title |
High titer production of canine distemper virus using cloned MDCK cell lines. |
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| Citation |
Korean J. Vet. Public Health 36:29-35(2012) |
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| Web pages |
https://agris.fao.org/agris-search/search.do?recordID=KR2013001818http://search.koreanstudies.net/thesis/thesis-view.asp?key=3054696 |
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| Abstract |
Canine distemper virus (CDV) causes serious and often fatal disease in
dogs. Currently, various cells or cell lines have been used to detect or
produce CDV. In order to set up the conditions, we separated two different
cell lines from Madin-Darby canine kidney (MDCK) and named as MDCK-F
(fibroblast-like) and MDCK-E (epithelial-like) by Na2EDDA treatment. CDV
seed virus was prepared using MDCK cells and inoculated into MDCK-F and
MDCK-E including MDCK with various ranges of multiplicity of infection
(MOI) to confirm the optimal amount of virus inoculation. The virus titer
of TCID50/ml was calculated by inoculation of serially diluted virus into
96-well plate of MDCK cells. The titer and cytopathic effect (CPE) in MDCK-E
were compared to those in MDCK-F. The titer of seed CDV was 1.24x10^6
TCID50/ml. Optimal MOI was about 0.1 for both MDCK-F and MDCK-E to obtain
highest titers of 10^8 TCID50/ml and 5x10^8 TCID50/ml respectively. CPE in
MDCK-E was shown 4 days after inoculation whether in MNCK-F 5~6 days after
inoculation. We can obtain highest titer of 5x10^8 TCID50/ml with 0.1 MOI
using MDCK-E. MDCK-E was more susceptible for CDV production than MDCK-F.
|
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| Cell lines |
CVCL_AQ31; MDCK-E [Korea] CVCL_AP75; MDCK-F |
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