| Publication number |
CLPUB00312 |
|---|
| Authors |
Liu Q.-G., Hu Y.-J., Shen L.-M. |
|---|
| Title |
Establishment of two cell lines from pupal ovary of Ectopus obliqua Warren (Lepidoptera, Geometridae). |
|---|
| Citation |
Shi Yan Sheng Wu Xue Bao 15:117-121(1982) |
|---|
| Abstract |
Two cell lines from pupal ovaries of Ectropis obliqua (SIE-EO-801, SIE-EO-803)
have been established since 1980. They have been transferred for more
than 73 passages and maintained for about one and a half year up to now.
They mainly consist of three cell types: epithelial-like, spindle-shaped
and round cells. The SIE-EO-801 line grows as monolayer culture and SIE-EO-803
line as both monolayer and suspension cultures. The type of
chromosomes reveals that it belongs to the karyotype of Lepidoptera. The
size of them are rather small and short, and the number varies greatly
from less than 30 to more than 200. Thus, it is impossible to count
accurately the number. In studying their characteristics, we found that
some differences exist between these two cell lines with respect to growth
behavior, morphology and enzyme activities. Ectropis obliqua is a major
pest of tea trees. The cell lines established from their ovary may thus be
used in the study of the biology of this insect.
|
|---|
| Cell lines |
CVCL_AV41; SIE-EO-801 CVCL_AV42; SIE-EO-803 |
|---|
