| Publication number |
CLPUB00284 |
|---|
| Authors |
Zhu D.-H., Ye X.-Z., Chen R.-M. |
|---|
| Title |
Establishment and some biological characteristics of a rat hepatoma cell line (CBRH-7919). |
|---|
| Citation |
Shi Yan Sheng Wu Xue Bao 13:113-117(1980) |
|---|
| Web pages |
https://en.cnki.com.cn/Article_en/CJFDTOTAL-SWSB198001010.htm |
|---|
| Abstract |
A rat hepatoma cell line (CBRH-7919) derived from the AFP positive
hepatoma of Wistar rat induced by the treatment of DENA has been
established in vitro. Cell suspension obtained by dispersing tumor tissue
with trypsin was inoculated into small bottles and cultivated as
stationary monolayer cultures, using medium RPMI-1640 supplemented with
20% fetal calf serum and the usual addition of antibiotics. This line of
cells has been successfully maintained in continuous cultivation for more
than 7 months (passed through 45 transfers). The cells are epithelial-like
in morphology with definite malignant characteristics by light and
electron microscopy. AFP was present in the cytoplasm as shown by
unlabelled immunoperoxidase technique. The homotransplantability of this
line of cells was high. Chromosome analysis showed a wide variation with a
mode around 70-80.
|
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| Cell lines |
CVCL_7176; CBRH-7919 |
|---|
