| Publication number |
CLPUB00273 |
|---|
| Authors |
Ginot L., Jeannesson P., Angiboust J.-F., Jardillier J.-C., Manfait M. |
|---|
| Title |
Interactions of adriamycin in sensitive and resistant leukemic cells: a comparative study by microspectrofluorometry. |
|---|
| Citation |
Studia Biophys. 104:145-153(1984) |
|---|
| Web pages |
https://jglobal.jst.go.jp/en/detail?JGLOBAL_ID=200902099133470377https://eurekamag.com/research/028/559/028559879.php |
|---|
| Abstract |
Based on the fluorescence properties of adriamycin (ADM), fluor-escence
emission spectra of this drug were recorded at the single cell level using
a highly sensitive microapectrofluorometer. Spectra were obtained from
micrometer regions in the cytoplasm or in the nucleus of single living
tumor cells which originated from culture or from mice ascitic tumors. In
sensitive ADM treated cells, ADM spectra show that the drug does not
interact during its cytoplasmic transit but intercalates in nuclear DNA in
a similar way to that obtained when ADM is combined With calf thymus DNA.
With ADM resistant leukemic cells, no intracellular fluorescence signal
was recorded either in the cytoplasm or in the nucleus which suggests an
inhibition of the drug uptake by resistant cells. By increasing
extracellular concentration of ADM, resistant cells allowed the drug to
penetrate but no signal of free or intercalated drug was observed.
|
|---|
| Cell lines |
CVCL_D573; K562/R7 |
|---|
