| Abstract |
Establishment of cell lines and tumorigenicity of human glioma cells were
investigated from the viewpoint of morphological differentiation and
anaplasia. For tissue culture, 82 human brain tumors including.34 gliomas
were used, and 3 glioma cell lines (designated NP-1, NP-2, NP-3) were
established. Cell line NP-1 was derived from an undifferentiated glioma
out of 2, whereas NP-2 from a differentiated astrocytic glioma with mild
anaplasia out of 19, NP-3 from one with marked anaplasia out of 2, and no
cell line from 11 differentiated gliomas without anaplasia. Plating
efficiency and colony formation in soft agar in NP-1 without S-100 and GFA
proteins were higher than those in NP-2 and NP-3 with only a few GFAP
staining cells. After 58 passages NP-1 (10^7 cells) began to produce
tumors subcutaneously in asplenic athymic (lasat) mice in 1.5 months, and
later in athymic (nude) mice in 3 to 8 months. But, after 122 passages
NP-3 produced the first tumor only in lasat mouse in 4 months, and NP-2
(7.5x10^7) had no tumorigenicity in either in 8 months. The histological
features were maintained by serial passaging in nude mice, and so the
tumor cells of heterotransplants of NP-1 had fewer 10nm filaments and
junctional complexes than those of NP-3. It was suggested that
establishment of cell lines and tumorigenicity of human glioma cells were
presumably related to the grade of differentiation: and anaplasia of
glioma cells, and especially to differentiation rather than anaplasia. In
addition, it would be extremely useful for the precise analysis of
tumorigenicity of human glioma cells to use lasat and nude mice together.
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