| Abstract |
The simple repetitive DNA of SME-1 cells at different passages was
detected by fluorescein labelled probe LZF-1. From the DNA fingerprints,
some results could be concluded: when the passage rised, the probability
of bands sharing, the maximum allelic frequency and the similarity
coefficient decreased between cells at the same passage and at the
different passage, while the mutation rate increased as variation of cells
strengthened. Therefore, to analyse the genetic variation of SME-1 cell line
by DNA fingerprinting was feasible. This paper seems to provide a good
detective method and genetical markers for clone culture of SME-1 cells,
and also provide a molecular basis for study of SME-1 cell line in cell
biology.
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