| Publication number |
CLPUB00232 |
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| Authors |
Suzuki M., Morita T., Hasegawa H., Watabe K., Miyazaki K., Ikeda M., Hanaoka K., Kawaguchi Y., Sakai O. |
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| Title |
Transformed cloned cells from microdissected tubules (proximal tubules). |
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| Citation |
Jikeikai Med. J. 39:119-126(1992) |
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| Web pages |
https://jglobal.jst.go.jp/en/detail?JGLOBAL_ID=200902063451579073 |
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| Abstract |
Renal tubules are composed of heterogeneous segments and different types
of cells. To obtain a homogeneous cell population of defined segment,
renal proximal convoluted tubules were cultured and transformed by plasmid
pSV3. The transformed single cells were grown to reach the 6th passage and
to become a homogeneous cell line. The cells were grown in FCS and
aminoglycoside enriched media and in hormone enriched media. Parathyr-oid
hormone, but not vasopressin, dose-dependently increased intracellular
free calcium as well as production of cAMP. Sodium dependent phosphate
transport was also active. Therefore, renal proximal tubule cells were
successfully transformed by the plasmid, which provide a useful material for
future study.
|
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| Cell lines |
CVCL_4679; PST-S2 |
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