Publication number |
CLPUB00218 |
Authors |
Zheng G.-L., Li C.-Y., Zhou H.-X., Li S.-W., Li G.-X., Xue M. |
Title |
Establishment of two new cell lines from the embryonic tissue of Helicoverpa armigera (Lepidoptera: Noctuidae) and their responses to baculovirus infection. |
Citation |
Acta Entomol. Sin. 53:167-174(2010) |
Web pages |
http://www.insect.org.cn/EN/Y2010/V53/I2/167 |
Abstract |
The development of insect cell lines plays a significant role in research
and application of virology and entomology. Two new cell lines, i.e.,
QB-Ha-E-1 and QB-Ha-E-5, were established from the embryonic tissue of
Helicoverpa armigera (Lepidoptera: Noctuidae). The cell lines had been
subcultured over 60 passages in TNM-FH medium supplemented with 10% fetal
bovine serum. Each cell line has two major morphological types, round
cells and spindle-shaped cells. DNA amplification fingerprinting (DAF)
analysis indicated that DNA profiles of the two cell lines were similar
but distinctly different from cell lines of several other insects,
suggesting that both originated from the embryonic tissue of H. armigera.
The cell doubling time of the 30th passages of QB-Ha-E-1 and QB-Ha-E-5
were 63.7 h and 66.9 h, respectively. Both the cell lines could be
infected by H. armigera single nucleopolyhedrovirus (HaSNPV) with the
infection rates of 86.6% and 56.5%, respectively, 4 d post infection (p.i.).
Approximately 15% of the two cell lines were infected by Mamestra
brassicae nucleopolyhedrovirus (MbNPV) 7 d p.i. The two cell lines had
differential responses to Autographa californica multiple
nucleopolyhedrovirus (AcMNPV) infection. The results from the fluorescent
staining with DAPI and electrophoresis of genomic DNA indicated that
QB-Ha-E-5 cell line had typical apoptotic response following AcMNPV infection,
but QB-Ha-E-1 cell line, with the AcMNPV infection rate of 55.3%, appeared
anti-apoptosis. Actinomycin D could induce apoptosis of the two cell lines
at the concentration of 1.25 mug/mL. Therefore, the two cell lines can be
used as ideal materials to study interactions between insect viruses and
insect cells.
|
Cell lines |
CVCL_0A14; QB-Ha-E-1 CVCL_0A15; QB-Ha-E-5 |