| Publication number |
CLPUB00210 |
|---|
| Authors |
Ueki K., Aizawa K., Tanaka S., Nishimaki T., Suzuki T. |
|---|
| Title |
Characterization of low- and high-level adriamycin-resistant human gastric cancer cell lines: flow cytometric studies and reversal effect of verapamil. |
|---|
| Citation |
Acta Med. Biol. 47:161-167(1999) |
|---|
| Web pages |
https://hdl.handle.net/10191/1824 |
|---|
| Abstract |
We have established two adriamycin (ADM)-resistant sublines with different
levels of drug resistance, MKN45R0.05 and MKN45R0.8, from the parental
MKN45 human gastric cancer cell line by exposure to stepwise increases in
ADM. MKN45R0.05 and MKN45R0.8 cells were 4.8-and 15-fold more resistant to
ADM than MKN45 cells, respectively. Flow cytometric (FCM) analysis
revealed that MKN45R0.8 cells had significantly lower accumulation of
cells in G_2M phase and higher efflux rate of intracellular ADM content
than MKN45 and MKN45R0.05 cells. There was no significant increase of
P-glycoprotein expression in both resistant sublines by FCM analysis.
Verapamil (VPM) showed stronger modifying effects on ADM sensitivity and
cell kinetics in MKN45R0.8 than MKN45R0.05 cells. These results indicate
that FCM analysis is useful for determining the level of drug resistance,
drug accumulation and P-glycoprotein expression in cancer cells, and that
the differential resistance-modifying activity of VPM depends on the level
of drug resistance.
|
|---|
| Cell lines |
CVCL_Z941; MKN45R0.05 CVCL_Z942; MKN45R0.08 |
|---|
