| Publication number |
CLPUB00207 |
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| Authors |
Bernhard H.P., Lienhard S., Regenass U. |
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| Title |
Isolation and characterization of mutant Drosophila cell lines. |
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| Citation |
(In book chapter) Invertebrate systems in vitro; Kurstak E., Maramorosch K., Dubendorfer A. (eds.); pp.13-26; Elsevier; Oxford; United Kingdom (1980) |
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| Web pages |
https://flybase.org/reports/FBrf0035336.html |
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| Abstract |
Since the first successful establishment of Drosophila cell lines by
Echalier and Ohanessian (1969) and Kakpakov et al. (1969), a large number
of permanent Drosophila cell lines have been established by several groups
but the collection of available cell lines is of limited genetic diversity
(Schneider and Blumenthal 1978). We have established a set of 14 different
cell lines that are derived from 10 well defined mutant Drosophila stocks.
The mutants were selected with respect to cytologically recognizable
marker chromosomes, allozyme variants, enzyme deficiencies, and
conditional lethality. The mutant cell lines were demonstrated to express
the respective mutant phenotypes at the cellular level and under in vitro
conditions. Cell lines carrying genetic markers and conditional lethal
mutations will prove useful for the isolation and identification of hybrid
Drosophila cells following somatic cell fusion (Bernhard 1976). The
conditional lethal cell lines will allow the study of in vitro mutagenesis
by determining the reversion frequency of defined mutations under
restrictive con-ditions. They provide further a critical and sensitive
assay system to study possible transformation in eucaryotic cells by means
of cloned gene sequences that might become available in the near future
(Gehring 1978).
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| Cell lines |
CVCL_Z901; E-Adh-n1 CVCL_WK71; E-Aldox-n1 CVCL_Z893; E-CS CVCL_Z902; E-E12[ts] mal[1] CVCL_Z903; E-FM1 CVCL_Z904; E-mal[1] CVCL_Z905; E-mal[F3] CVCL_Z894; E-OR CVCL_Z906; E-r[1] CVCL_Z907; E-r[36] |
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