| Publication number |
CLPUB00206 |
|---|
| Authors |
Pant U., Mascarenhas A.F., Jagannathan V. |
|---|
| Title |
In vitro cultivation of a cell line from embryonic tissue of potato tuber moth Gnorimoschema operculella (Zeller). |
|---|
| Citation |
Indian J. Exp. Biol. 15:244-245(1977) |
|---|
| Web pages |
https://agris.fao.org/agris-search/search.do?recordID=US201302975958https://eurekamag.com/research/016/092/016092626.php |
|---|
| Abstract |
Cells from embryonic tissue of G. operculella have been grown in vivo on
Mitsuhashi and Maramorosch's medium (containing yeast extract, lactalbumin
hydrolysate and foetal bovine serum) for about 600 days and subcultured 77
times. Cells consist of morphologically different cell types. They grow
well both as a monolayer attached to glass or in suspension culture on a
shaker. Cell doubling time in stationary culture is about 48 hr. Cells
also grow well on medium containing bovine serum albumin instead of foetal
bovine serum and have been subcultured 16 times on this medium without
decrease in growth rate.
|
|---|
| Cell lines |
CVCL_Z988; G01-874 |
|---|
