Publication number |
CLPUB00196 |
Authors |
Mitsuhashi J. |
Title |
A new continuous cell line from the larval fat bodies of the mulberry tiger moth, Spilosoma imparilis (Lepidoptera: Arctiidae). |
Citation |
Bull. Natl. Inst. Seric. Entomol. Sci. 11:1-8(1994) |
Web pages |
https://agris.fao.org/agris-search/search.do?recordID=JP9503033https://eurekamag.com/research/008/051/008051671.php |
Abstract |
The author established a new continuous cell line from the larval fat
bodies of Spilosoma imparilis. In the early stage of the process of
establishing the cell line, the culture experienced critical conditions
several times. However, after a cell suspension by pipetting was
introduced into a newly setted-up primary culture of the same species and
tissues, the transferred cells recovered their ability of cell division in
about half a month. In this cell line many cells showed strong anchorage-
dependency. Population doubling time of this cell line was about 3.5 days.
Chromosome numbers frequently observed were around 100 and 190. Five
isozyme patterns were identified. The cell line was highly susceptible to
Spilosoma imparilis nuclear polyhedrosis virus and Hyphantria cunea
nuclear polyhedrosis virus.
|
Cell lines |
CVCL_Z546; FFPRI-SpIm-2AM |