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Cellosaurus publication CLPUB00196

Publication number CLPUB00196
Authors Mitsuhashi J.
Title A new continuous cell line from the larval fat bodies of the mulberry tiger moth, Spilosoma imparilis (Lepidoptera: Arctiidae).
Citation Bull. Natl. Inst. Seric. Entomol. Sci. 11:1-8(1994)
Web pages https://agris.fao.org/agris-search/search.do?recordID=JP9503033
https://eurekamag.com/research/008/051/008051671.php
Abstract The author established a new continuous cell line from the larval fat bodies of Spilosoma imparilis. In the early stage of the process of establishing the cell line, the culture experienced critical conditions several times. However, after a cell suspension by pipetting was introduced into a newly setted-up primary culture of the same species and tissues, the transferred cells recovered their ability of cell division in about half a month. In this cell line many cells showed strong anchorage- dependency. Population doubling time of this cell line was about 3.5 days. Chromosome numbers frequently observed were around 100 and 190. Five isozyme patterns were identified. The cell line was highly susceptible to Spilosoma imparilis nuclear polyhedrosis virus and Hyphantria cunea nuclear polyhedrosis virus.
Cell lines CVCL_Z546; FFPRI-SpIm-2AM