| Publication number |
CLPUB00187 |
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| Authors |
Sohi S.S., Ennis T.J. |
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| Title |
Chromosomal characterization of cell lines of Neodiprion lecontei (Hymenoptera; Diprionidae). |
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| Citation |
Proc. Entomol. Soc. Ont. 112:45-48(1981) |
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| Web pages |
https://cfs.nrcan.gc.ca/publications?id=22418https://agris.fao.org/agris-search/search.do?recordID=CA19830930182 |
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| Abstract |
The redheaded pine sawfly, Neodiprion Lecontei (Fitch), is an economically
important pest of young pines in eastern Canada and the U.S.A. (Wilson and
Reeks 1967). To facilitate study of a nuclear polyhedrosis virus that is
very virulent for this sawfly (Bird 1961), ten sawfly cell lines were
isolated from embryonic tissues. We have undertaken a chromosomal study of
these cell lines to confirm their sawfly origin, and to determine whether
or not they could be distinguished from each other on the basis of their
chromosome complement. There are several reports (Herrick et al. 1970;
Greene et al. 1972) of incorrect identification of vertebrate and insect
cell lines because of cross contamination and/or mislabeling. We carry
several lepidopteran cell lines which could be a source of contamination
for new cell lines. Chromosomal characteristics of the lepidopteran cell
lines have been reported previously (Ennis and Sohi 1976). In this note we
analyze the chromosomal complement of the sawfly cell lines.
|
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| Cell lines |
CVCL_GZ68; FPMI-NL-1 CVCL_Z493; FPMI-NL-2 CVCL_Z498; FPMI-NL-4 CVCL_Z491; FPMI-NL-10 CVCL_Z492; FPMI-NL-18 CVCL_Z494; FPMI-NL-21 CVCL_Z495; FPMI-NL-22 CVCL_GZ69; FPMI-NL-27 CVCL_Z496; FPMI-NL-28 CVCL_GZ70; FPMI-NL-31 |
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