| Publication number |
CLPUB00186 |
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| Authors |
Sohi S.S., Lalouette W., MacDonald J.A., Gringorten J.L., Budau C.B., Roden P.M. |
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| Title |
Establishment of continuous midgut cell lines of spruce budworm (Lepidoptera: Tortricidae). |
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| Citation |
In Vitro Cell. Dev. Biol. Anim. 29:56A-56A(1993) |
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| Web pages |
https://www.jstor.org/stable/4294015 |
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| Abstract |
The spruce budworm, Choristoneura fumiferana, is the most destructive
forest insect pest in Canada and the northern United States. We have
established its four continuous midgut cell lines for use in developing
biorational control agents for this insect. The midguts were removed from
surface sterilized insects. After cleaning, they were chopped into small
pieces, and treated with 0.05% trypsin. The tissue fragments were removed
by centrifugation, resuspended in growth medium, transferred to 25-cm2
plastic tissue culture flasks, and were kept at 28 Celsius. Three different
media (Grace's, EX-CELL 400, and SF-900) supplemented with 10-12% fetal
bovine serum were used. The cell lines have been subcultured 50-125 times.
Their species identity has been confirmed by isozyme analysis. The cells
are of different sizes and shapes, and grow attached to the substrate.
Electron microscopy has revealed that the cultures have mixed populations
of midgut cells and other cell types. The cells have been freeze-preserved
in liquid nitrogen and successfully revived. Some of the cell lines
replicate the spruce budworm nuclear polyhedrosis virus and some are
susceptible to certain delta-endotoxin proteins of Bacillus thuringiensis.
|
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| Cell lines |
CVCL_WV83; FPMI-CF-200 CVCL_Z469; FPMI-CF-203 CVCL_WV84; FPMI-CF-204 CVCL_WV85; FPMI-CF-205 |
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