| Publication number |
CLPUB00184 |
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| Authors |
Inoue H., Taniai K., Kobayashi J. |
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| Title |
Establishment and characterization of substrate-depending cell lines of Bombyx mori. |
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| Citation |
Bull. Natl. Inst. Seric. Entomol. Sci. 1:13-25(1990) |
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| Web pages |
https://agris.fao.org/agris-search/search.do?recordID=JP19930108537https://eurekamag.com/research/007/312/007312699.php |
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| Abstract |
A substrate-depending cell line of Bombyx mori and its cloned cell line
were established and characterized for virus cloning in a plaque assay in
order to develop a baculovirus-expression system. The cell line designated
as SES-BoMo-15A, which is a mixture of floating and adherent cells, was
cultured in a MGM-448 medium containing a high concentration of TC-AM-1
antibiotics. Although many cells were damaged and died, the remaining
cells gradually recovered their growth activity when they were cultured in
a medium containing a normal concentration of antibiotics, and about 7
months later adherent cells could be subcultured. This substrate-depending
cell line was named SES-BoMo-15 AII. The BoMo-15 AII cells were firmly
attached to the base of the culture vessel, and were usually detached by
treatment with pancreata in following pipetting. The population doubling
time was about 4 days. The cell line was highly susceptible to B. mori
nuclear polyhedrosis virus (NPV) and also to both B. mori cytoplasmic
polyhedrosis virus (CPV) 'A strain and Euxoa scandens CPV. Furthermore a
cell line, named NISES-BoMo-15 AIIc, which was cloned by a colonial
cloning method from SES-BoMo-15 AII exhibited almost the same
characteristics as the original cell line.
|
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| Cell lines |
CVCL_Z420; NISES-BoMo-15AIIc CVCL_Z108; SES-BoMo-15A CVCL_Z419; SES-BoMo-15AII |
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