| Abstract |
Grace established a cell line from axenically grown larva of Aedes aegypti
about to pupate by employing a culture medium containing haemolymph of the
moth, Anthercea eucalypti. However, his further efforts to establish more
cell lines from A. aegypti were not successful. Kitamura cultured ovarian
tissue of three species of mosquitoes, Culex molestus, Aedes albopictus
and Aedes aegypti, using a culture medium consisting of medium 199 and
calf serum. He was able to subculture the cells of Aedes up to the eighth
passage. Many other workers have also attempted to culture and subculture
mosquito cells without much success. This communication reports the
successful establishment of three cell lines of A. albopictus and two of A.
aegypti using a culture medium without insect haemolymph.
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