| Publication number |
CLPUB00176 |
|---|
| Authors |
Pant U., Athawale S.S., Basu A., Banerjee K. |
|---|
| Title |
A new cell line established from pupal ovaries of Spodoptera litura (F.) (Lepidoptera: Noctuidae). |
|---|
| Citation |
Indian J. Exp. Biol. 36:195-198(1998) |
|---|
| Abstract |
Steady growth of cells from pupal ovaries of tobacco cutworm, S. litura
was observed 107 days after initiation. Three cell types, viz. epithelial-
like cells (91%), fibroblast-like cells (7%) and giant cells (2%) could be
distinguished. The cells grew loosely attached to the glass/plastic
surface and could be easily dispersed by pipetting. The cells could be
cryopreserved and at present are at 126th passage level. The chromosome
number was high ranging from 41-200 and even more. Electron microscopy
indicated the presence of virus-like particles in the nuclei of a
fraction of the cell population. The cells were susceptible to nuclear
polyhedrosis virus, Autographa californica.
|
|---|
| Cell lines |
CVCL_Z351; NIV-SU-992 |
|---|
