| Publication number |
CLPUB00169 |
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| Authors |
Petcharawan O., Mongkolpoch K., Belloncik S. |
|---|
| Title |
Establishment of cell line derived from embryonic tissue of the diamondback moth, Plutella xylostella (L.). |
|---|
| Citation |
KMITL Sci. Tech. J. 6:56-66(2006) |
|---|
| Web pages |
https://li01.tci-thaijo.org/index.php/cast/article/view/94021 |
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| Abstract |
A new cell line designated KMITL-PX-E1 has been established from embryonic
tissue of the diamondback moth, Plutella xylostella (L.). The primary
cultures were seeded in TNM-FH medium supplemented with 20% fetal bovine
serum and incubated at 28 Celsius. After the 18th passage, the cultures
were adapted and maintained in TNM-FH medium supplemented with 10% fetal
bovine serum. The cell line consisted of a mixture of two cell types,
spindle-shaped cells and epithelial-like cells, both of them grew as
attached monolayer. The population doubling time at the 2nd and the 18th
passages were 110.59 and 61.22 hrs, respectively. The chromosome numbers
varied from 6-124. Random amplified polymorphic DNA profile analysis
showed that the diamondback moth cell line (KMITL-PX-E1) profile differed
from the profiles of the beet armyworm (SE-1) and the American cotton
bollworm (KMITL-HA-E1) cell lines maintained in the laboratory. KMITL-PX-E1
cell line supported replication of Autographa californica (AcMNPV) and
Exoa scandens cytoplasmic polyhedrosis virus (EsCPV). However, this cell
line was sensitive to Bacillus thuringiensis toxin.
|
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| Cell lines |
CVCL_Z386; KMITL-PX-E1 |
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