Publication number |
CLPUB00164 |
Authors |
Deshmukh C.G., Bahekar R.S. |
Title |
Comparative in vitro replication and serial passaging of BMNPV in the DZNU-BM-12 and other cell lines. |
Citation |
Int. J. Sci. Res. Pub. 3:3.1-3.3(2013) |
Web pages |
https://www.ijsrp.org/research-paper-0313/ijsrp-p1588.pdf |
Abstract |
The newly established ovarian cell line of Bombyx mori, DZNU-Bm-12 was
tested for its susceptibility to homologous nucleopolyhedrovirus, BmNPV.
The BmNPV was serially passaged in the cell line for five times along with
the other four cell lines Bm-1, Bm-16 and Bm-17 which is established in
our laboratory and Bm-5 is a widely used cell line. All the cell lines are
susceptible to BmNPV. The overall range of BmNPV infection during serial
passaging was more than 90% was observed in Bm-1, Bm-5 and Bm-16, whereas
in Bm-12 and Bm-17 it was between 77 and 89%. The average number of
OBs/infected cell was 16-19 in Bm-1, Bm-5 and Bm-16 but in Bm-12 it was
9-13 and only 7-8 in Bm-17.
|
Cell lines |
CVCL_0A00; Bm5 CVCL_Z082; DZNU-Bm-1 CVCL_Z115; DZNU-Bm-12 CVCL_Z304; DZNU-Bm-16 CVCL_Z116; DZNU-Bm-17 |