| Abstract |
Larval hemocytes of Malacosoma neustria testacea (Lepidoptera,
Lasiocampidae) were cultured in MGM-450 medium added with 5% fetal bovine
serum (FBS) and Antheraea pernyi hemolymph. Out of thirty primary
cultures set up, a continuous cell line was obtained, and designated
FRI-MntH-520A, Cells of this cell line could grow in MGM-450 medium fortified
with 10% fetal bovine serum alone, and the population doubling time was
6.93 days. Spherical cells were predominant but sometimes odd-shaped cells
appeared, Karyotype of the cell line was typical of lepidopteran cell
lines and consisted of numerous small chromosomes. The cell line could be
discriminated from other lepidopteran cell lines by comparing the patterns
of isozymes of several enzymes. M. neustria testacea nuclear polyhedrosis
virus (NPV) infected the cell line whereas Autographa californica,
Malacosoma distria, and Lambdina fiscellaria somniaria NPVs did not.
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