| Publication number |
CLPUB00147 |
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| Authors |
Faris R.A., Monfils B.A., Brown J., Yang L., Affigne S., Marceau N., Hixson D.C. |
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| Title |
H5D.7, an azo dye induced transplantable hepatocellular carcinoma cell line that possesses multipotential stem cell properties. |
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| Citation |
Proc. Am. Assoc. Cancer Res. 31:156.931-156.931(1990) |
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| Web pages |
https://eurekamag.com/research/031/632/031632203.php |
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| Abstract |
Earlier studies suggested that oval cells were a progenitor of PHC during
methyl-4-dimethylaminobenzene (3-MeDAB) induced liver cancer. Subsequent
investigations have shown that the morphologically-defined oval cell
population is phenotypically heterogeneous and contains cells that can
differentiate into either bile ducts or hepatocytes. In the present
investigation, we have examined the antigenic and biological properties of
a transplantable hepatocellular carcinoma, designated H5D, which was
established in culture from a 3-MeDAB primary tumor. Limiting dilution
cloning of H5D produced several cell lines that expressed the oval cell
antigens, OC.2 and OC.3. Inununofluorescence analysis demonstrated that at
confluency, the clone designated H5D.7 contained cells expressing oval
cell (OC.2, OC.3, OV-1 and OV-6), hepatocyte (cell CAM 105 and
dipeptitlylpeptidase IV), and epithelial (desmoplakin I) markers. After
continuous passage. the H5D.7 clone remained heterogenous with respect to
the expression of oval cell antigens but showed more uniform expression of
hepatocyte markers. Numerous attempts to isolate phenotypically
homogeneous clones from H5D.7 were unsuccessful suggesting that these
cells undergo antigenic differentiation as they grow in culture. This idea
is supported by the observation that following each passage, hepatocyte
antigens were present in only a subpopulations of cells. When H5D.7 was
transplanted subcutanously in rats the tumors were histologically complex
and contained areas of moderately-differentiated hepatocelluar carcinoma,
intestinal metaplasia and ductal structures. Taken together, these
observations suggest that the H5D.7 clone is comprised of multipotential
cells.
|
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| Cell lines |
CVCL_X360; H5D.7 |
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