| Publication number |
CLPUB00137 |
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| Authors |
Chen S.-N., Ueno Y., Kou G.-H. |
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| Title |
A cell line derived from Japanese eel (Anguilla japonica) kidney. |
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| Citation |
Proc. Natl. Sci. Counc. Repub. China, B, Life Sci. 6:93-100(1982) |
|---|
| Abstract |
A permanent cell line designated EK-1 was developed from Japanese eel (A.
japonica) kidney. The cell line was passed for 110 transfers in a period
of 18 mo. in Leibovitz's L-15 medium plus 10% fetal calf serum at a
temperature of 32 +- 1 degree Celsius. The EK-1 cell line consists of
epitheloid cells with micro-or macro-nuclei and fibroblastic cells.
Multinucleated epitheloid cells were present in most of the cultures. The
results of chromosome analysis for the EK-1 cell line revealed that the
chromosome numbers counted from 100 metaphase cells show a bimodal
distribution ranging from 21-62, with a modal number of 38, which is
similar to that of normal eel somatic ells. When subjected to low seeding
densities, the EK-1 cells showed a low plating efficiency, which might
result from the non-transformation of the cells. The cells were
susceptible to Eel Virus America (EVA), Eel Virus Europe (EVE) or Eel
Virus Europe X (EVEX). The EK-1 cell line was demonstrated to be free of
bacterial, fungal and mycoplasmic contamination.
|
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| Cell lines |
CVCL_8348; EK-1 |
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